Cynomolgus SFRP2 Protein (SRP-CM102)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Leu25-Cys295

Catalog: SRP-CM102

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Product Description Recombinant Cynomolgus SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295.

Product Background

 As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients.

Product Category

 Recombinant Protein

Target

 SFRP2

Gene/Protein ID

 G8F2Z7

Species

 Cynomolgus

Exact Sequence

 Leu25-Cys295
Molecular Weight The protein has a predicted MW of 32.16 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Cynomolgus SFRP2 is greater than 95% as determined by SEC-HPLC.
Cynomolgus SFRP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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