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Cynomolgus SFRP2 Protein (SRP-CM102) Summary
Product Description |
Recombinant Cynomolgus SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295. |
Product Background |
As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus SFRP2 Protein (SRP-CM102) Specifications/Details
Protein |
SFRP2 |
Synonyms |
sFRP-2; SARP-1; Sarp1; Sdf5; Sfrp2 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Leu25-Cys295 |
Molecular Weight |
The protein has a predicted MW of 32.16 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Cynomolgus SFRP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Cynomolgus SFRP2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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