Product Description |
Recombinant Cynomolgus SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295. |
Product Background |
As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients. |
Product Category |
Recombinant Protein |
Target |
SFRP2 |
Synonyms |
sFRP-2; SARP-1; Sarp1; Sdf5; Sfrp2 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Leu25-Cys295 |
Molecular Weight |
The protein has a predicted MW of 32.16 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Cynomolgus SFRP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Cynomolgus SFRP2 is greater than 95% as determined by SEC-HPLC. |
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