- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Cynomolgus SG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Phe20-Leu468
Product Description |
Recombinant Cynomolgus SG3/Secretogranin 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe20-Leu468. |
Product Background |
Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
SG3/Secretogranin 3 |
Synonyms |
Secretogranin-3; Secretogranin III; SCG3; SGIII; UNQ2502/PRO5990 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Phe20-Leu468 |
Molecular Weight |
The protein has a predicted MW of 52.14 kDa. Due to glycosylation, the protein migrates to 58-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus SG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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