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Cynomolgus SG3/Secretogranin 3 Protein, Ultra Low Endotoxin (SGS-CM101-UL) Summary
Product Description |
Recombinant Cynomolgus SG3/Secretogranin 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe20-Leu468. |
Product Background |
Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus SG3/Secretogranin 3 Protein, Ultra Low Endotoxin (SGS-CM101-UL) Specifications/Details
Protein |
SG3/Secretogranin 3 |
Synonyms |
Secretogranin-3; Secretogranin III; SCG3; SGIII; UNQ2502/PRO5990 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Phe20-Leu468 |
Molecular Weight |
The protein has a predicted MW of 52.14 kDa. Due to glycosylation, the protein migrates to 58-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus SG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus SG3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. |
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Cynomolgus SG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus SG3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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