Cynomolgus Siglec-15/CD33L3 Protein (SIG-CM415)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus Siglec-15, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met60-Thr322

Catalog: SIG-CM415

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Product Description Recombinant Cynomolgus Siglec-15/CD33L3 Protein is expressed from Expi293 with His and Avi tag at the C-terminal.; It contains Met60-Thr322.
Product Category Recombinant Protein
Target Siglec-15/CD33L3
Synonyms CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
Gene/Protein ID XP_005586866.2
Species Cynomolgus
Exact Sequence Met60-Thr322
Molecular Weight The protein has a predicted MW of 29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus Siglec-15, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PB, 500mM NaCl, 0.1M L-arginine (pH 6.0). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in 20mM PB, 0.5M NaCl, 5% glycerol, 0.1M L-Arginine (pH 6.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus Siglec-15, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
The purity of Cynomolgus Siglec-15 is greater than 95% as determined by SEC-HPLC.
Cynomolgus Siglec-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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