Cynomolgus SIRP alpha/CD172a Protein (SRP-CM172)
Catalog: SRP-CM172
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| Product Description | Recombinant Cynomolgus SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg370. |
Product Background |
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. |
Product Category |
Recombinant Protein |
Target |
SIRP alpha/CD172a |
| Synonyms | CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; SIRP alpha; SIRPA; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3 |
Gene/Protein ID |
NP_001271679.1 |
Species |
Cynomolgus |
Exact Sequence |
Glu31-Arg370 |
| Molecular Weight | The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA (QC Test). The affinity constant of 63.27 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA (QC Test). |
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The purity of Cynomolgus SIRP alpha is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus SIRP alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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