Name: Cynomolgus SPARC Protein (SPA-CM101)
Brand: KactusBio
SKU: SPA-CM101-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus SPARC, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 67.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ala18-Ile303

Catalog #SPA-CM101

Size

100UG

500UG

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Product Description	Recombinant Cynomolgus SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303.
Product Background	Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
Product Category	Recombinant Protein / Antibody discovery

Protein	SPARC
Synonyms	SPARC; BM-40; ON; EGM_15571
Accession	G7P8R2
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Ala18-Ile303
Molecular Weight	The protein has a predicted MW of 33.82 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus SPARC, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 67.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Cynomolgus SPARC, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 67.8ng/ml determined by ELISA.
	Cynomolgus SPARC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Cynomolgus SPARC is greater than 95% as determined by SEC-HPLC.