Product Description |
Recombinant Cynomolgus SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303. |
Product Background |
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
SPARC |
Synonyms |
SPARC; BM-40; ON; EGM_15571 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Ala18-Ile303 |
Molecular Weight |
The protein has a predicted MW of 33.82 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus SPARC, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 67.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Cynomolgus SPARC, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 67.8ng/ml determined by ELISA. |
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Cynomolgus SPARC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus SPARC is greater than 95% as determined by SEC-HPLC. |
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