Cynomolgus SPARC Protein (SPA-CM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus SPARC, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala18-Ile303

Catalog: SPA-CM101

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Product Description	Recombinant Cynomolgus SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile303.
Product Background	Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
Product Category	Recombinant Protein

Target	SPARC
Gene/Protein ID	G7P8R2
Species	Cynomolgus
Exact Sequence	Ala18-Ile303
Molecular Weight	The protein has a predicted MW of 33.82 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus SPARC, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus SPARC, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA.
	The purity of Cynomolgus SPARC is greater than 95% as determined by SEC-HPLC.
	Cynomolgus SPARC on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.