Cynomolgus/Rhesus macaque TIGIT Protein (TIG-CM110)
Cynomolgus/Rhesus macaque TIGIT Protein (TIG-CM110)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met22-Ile141
Catalog #TIG-CM110
Regular price $270.00
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Product Description
Recombinant Cynomolgus/Rhesus macaque TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met22-Ile141.
Product Background
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Product Category
Recombinant Protein
Target
TIGIT
Synonyms
TIGIT; VSIG9; VSTM3; WUCAM
Accession
Species
Cynomolgus/Rhesus macaque
Biotinylated
no
Amino Acid Range
Met22-Ile141
Molecular Weight
The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus/Rhesus macaque TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus/Rhesus macaque TIGIT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA.
Cynomolgus/Rhesus macaque TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque TIGIT is greater than 95% as determined by SEC-HPLC.

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