Cynomolgus TIGIT Protein (TIG-CM110)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus TIGIT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 30.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met89-Ile208

Catalog: TIG-CM110

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Product Description Recombinant Cynomolgus TIGIT Protein is expressed from Expi293 with His tag at the C-terminal.It contains Met89-Ile208.
Product Category Recombinant Protein
Target TIGIT
Synonyms TIGIT; VSIG9; VSTM3; WUCAM
Gene/Protein ID XP_015300911.1
Species Cynomolgus
Exact Sequence Met89-Ile208
Molecular Weight The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus TIGIT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 30.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus TIGIT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 30.3ng/ml determined by ELISA.
The purity of Cynomolgus TIGIT is greater than 95% as determined by SEC-HPLC.
Cynomolgus TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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