Cynomolgus TNFR1/CD120a/TNFRSF1A Protein (TNF-CM1R1)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus TNFR1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu30-Thr211
Catalog #TNF-CM1R1
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Product Description
 Recombinant Cynomolgus TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Thr211.
Product Background
 Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.
Product Category
 Recombinant Protein
Target
 TNFR1/CD120a/TNFRSF1A
Synonyms
 TNF-R1; TNF-RI; FPF; TBP1; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNFR1-d2
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Leu30-Thr211
Molecular Weight
 The protein has a predicted MW of 21.62 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus TNFR1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus TNFR1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test).
Cynomolgus TNFR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus TNFR1 is greater than 95% as determined by SEC-HPLC.

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