Cynomolgus TNFR1/CD120a/TNFRSF1A Protein (TNF-CM1R1)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus TNFR1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu30-Thr211

Catalog: TNF-CM1R1

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Product Description Recombinant Cynomolgus TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Thr211.

Product Background

 Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.

Product Category

 Recombinant Protein

Target

 TNFR1/CD120a/TNFRSF1A

Gene/Protein ID

 A0A7N9CVF2

Species

 Cynomolgus

Exact Sequence

 Leu30-Thr211
Molecular Weight The protein has a predicted MW of 21.62 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Cynomolgus TNFR1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus TNFR1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test).
The purity of Cynomolgus TNFR1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus TNFR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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