SPR sensorgram showing concentration-dependent binding response over time, used for analyzing molecular interaction kinetics in real time.
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus TNFR1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu30-Thr211
Catalog #TNF-CM1R1
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Cynomolgus TNFR1/CD120a/TNFRSF1A Protein (TNF-CM1R1) Summary

Product Description

Recombinant Cynomolgus TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Thr211.

Product Background

Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Cynomolgus TNFR1/CD120a/TNFRSF1A Protein (TNF-CM1R1) Specifications/Details

Protein
TNFR1/CD120a/TNFRSF1A
Synonyms
TNF-R1; TNF-RI; FPF; TBP1; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNFR1-d2
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Leu30-Thr211
Molecular Weight
The protein has a predicted MW of 21.62 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus TNFR1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus TNFR1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus TNFR1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test).
Cynomolgus TNFR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus TNFR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus TNFR1 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus TNFR1 is greater than 95% as determined by SEC-HPLC.
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