Cynomolgus TNFR2/CD120b/TNFR1B Protein (TNF-CM1R2)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus TNFR2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 14ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu23-Asp257

Catalog: TNF-CM1R2

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Product Description Recombinant Cynomolgus TNFR2/CD120b/TNFR1B Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Leu23-Asp257.
Product Category Recombinant Protein
Target TNFR2/CD120b/TNFR1B
Synonyms CD120b; Etanercept; p75TBPII; p75TNFR; TNF RII; TNF-R2; TNF-R75; TNFR80; TNFRSF1B; TNFR2; TBPII; TNFBR; TNFR1B
Gene/Protein ID A0A2K5VEV7
Species Cynomolgus
Exact Sequence Leu23-Asp257
Molecular Weight The protein has a predicted MW of 26.1 kDa. Due to glycosylation, the protein migrates to 49-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus TNFR2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 14ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus TNFR2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 14ng/ml determined by ELISA.
The purity of Cynomolgus TNFR2 is greater than 95% as determined by SEC-HPLC.
Cynomolgus TNFR2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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