- Express System: HEK293
- Product Tag: C-His
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: Immobilized Cynomolgus TREM1, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human PGLYRP1, hFc Tag with the EC50 of 1.14 ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Thr21-Pro201
Product Description |
Recombinant Cynomolgus TREM1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr21-Pro201. |
Product Background |
TREM1 (Triggering Receptor Expressed on Myeloid Cells 1) is a pro-inflammatory receptor expressed by phagocytes, which can also be released as a soluble molecule (sTREM1). The roles of TREM1 and sTREM1 in liver infection and inflammation are not clear. |
Product Category |
Recombinant Protein |
Target |
TREM1 |
Synonyms |
CD354; TREM-1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Thr21-Pro201 |
Molecular Weight |
The protein has a predicted MW of 23.76 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus TREM1, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human PGLYRP1, hFc Tag with the EC50 of 1.14 ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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