Cynomolgus TSLP (R127A, R130S) Protein (TSP-CM102)

Immobilized Human TSLPR, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Cynomolgus TSLP, His Tag with the EC50 of 2.2ng/ml determined by ELISA.

Immobilized Cynomolgus TSLP (R127A, R130S), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.

Cynomolgus TSLP (R127A, R130S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Cynomolgus TSLP (R127A, R130S), His Tag with an affinity constant of 8.14 nM as determined in BLI assay.

Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus TSLP (R127A, R130S), His Tag with an affinity constant of 0.32 nM as determined in BLI assay.

The purity of Cynomolgus TSLP (R127A, R130S) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TSLPR, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Cynomolgus TSLP, His Tag with the EC50 of 2.2ng/ml determined by ELISA.
Amino Acid Range: Tyr29-Gln159(R127A, R130S)

Catalog #TSP-CM102

Price

$363⁰⁰

Size

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Cynomolgus TSLP (R127A, R130S) Protein (TSP-CM102) Summary

Product Description

Recombinant Cynomolgus TSLP (R127A, R130S) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr29-Gln159 (R127A, R130S).

Product Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Cynomolgus TSLP (R127A, R130S) Protein (TSP-CM102) Specifications/Details

Protein	TSLP
Synonyms	thymic stromal lymphopoietin; TSLP
Accession	A0A7N9CAT7
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Tyr29-Gln159(R127A, R130S)
Molecular Weight	The protein has a predicted MW of 16.65 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TSLPR, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Cynomolgus TSLP, His Tag with the EC50 of 2.2ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human TSLPR, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Cynomolgus TSLP, His Tag with the EC50 of 2.2ng/ml determined by ELISA.
	Immobilized Cynomolgus TSLP (R127A, R130S), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
	Cynomolgus TSLP (R127A, R130S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Cynomolgus TSLP (R127A, R130S), His Tag with an affinity constant of 8.14 nM as determined in BLI assay.
	Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus TSLP (R127A, R130S), His Tag with an affinity constant of 0.32 nM as determined in BLI assay.
	The purity of Cynomolgus TSLP (R127A, R130S) is greater than 95% as determined by SEC-HPLC.