Product Description |
Recombinant Cynomolgus TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Lys231. |
Product Background |
Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2). |
Product Category |
Recombinant Protein |
Target |
TSLPR |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln23-Lys231 |
Molecular Weight |
The protein has a predicted MW of 25.42 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus TSLPR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus TSLPR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. |
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Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). |
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Cynomolgus TSLPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus TSLPR is greater than 95% as determined by SEC-HPLC. |
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