Cynomolgus uPAR/PLAUR Protein (PAR-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus uPAR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu23-Gly305
Catalog #PAR-CM101
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Product Description
 Recombinant Cynomolgus uPAR/PLAUR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly305.
Product Background
 The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
Product Category
 Recombinant Protein
Target
 uPAR/PLAUR
Synonyms
 U-PAR; uPAR; CD87; PLAUR; MO3; UPAR
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Leu23-Gly305
Molecular Weight
 The protein has a predicted MW of 32.69 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus uPAR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus uPAR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).
Cynomolgus uPAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus uPAR is greater than 95% as determined by SEC-HPLC.

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