Cynomolgus uPAR/PLAUR Protein (PAR-CM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus uPAR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu23-Gly305

Catalog: PAR-CM101

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Product Description Recombinant Cynomolgus uPAR/PLAUR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly305.

Product Background

The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.

Product Category

Recombinant Protein

Target

uPAR/PLAUR
Accession

Species

Cynomolgus

Amino Acid Range

Leu23-Gly305
Molecular Weight The protein has a predicted MW of 32.69 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus uPAR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus uPAR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test).
The purity of Cynomolgus uPAR is greater than 95% as determined by SEC-HPLC.
Cynomolgus uPAR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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