• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu25-Gly168
Catalog #PD1-FM101
Price
Regular price $34500
Regular price Sale price $34500
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Feline PD-1/PDCD1 Protein (PD1-FM101) Summary

Product Description

 Recombinant Feline PD-1/PDCD1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu25-Gly168.

Product Background

 Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.

Feline PD-1/PDCD1 Protein (PD1-FM101) Specifications/Details

Protein
 PD-1/PDCD1
Synonyms
 PDCD1; PD1; CD279; SLEB2; PD-1
Accession
Species
 Feline
Biotinylated
 no
Amino Acid Range
 Leu25-Gly168
Molecular Weight
 The protein has a predicted MW of 17.75 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Feline PD-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Feline PD-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Feline PD-1 is greater than 95% as determined by SEC-HPLC. The purity of Feline PD-1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...