Feline PD-1/PDCD1 Protein (PD1-FM201)

Immobilized Biotinylated Human PD-L1, His Tag (Cat. PDL-HM410B) at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Feline PD-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.

Feline PD-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Feline PD-1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human PD-L1, His Tag (Cat. PDL-HM410B) at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Feline PD-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
Amino Acid Range: Leu25-Gly168

Catalog #PD1-FM201

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Feline PD-1/PDCD1 Protein (PD1-FM201) Summary

Product Description

Recombinant Feline PD-1/PDCD1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Leu25-Gly168.

Product Background

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.

Feline PD-1/PDCD1 Protein (PD1-FM201) Specifications/Details

Protein	PD-1/PDCD1
Synonyms	PDCD1; PD1; CD279; SLEB2; PD-1
Accession	A9YUA6
Species	Feline
Biotinylated	no
Amino Acid Range	Leu25-Gly168
Molecular Weight	The protein has a predicted MW of 42.09 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human PD-L1, His Tag (Cat. PDL-HM410B) at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Feline PD-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human PD-L1, His Tag (Cat. PDL-HM410B) at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Feline PD-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
	Feline PD-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Feline PD-1 is greater than 95% as determined by SEC-HPLC.