Felis catus TNF alpha-Protein (active trimer) (TNF-FM10A)

Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA.

Felis catus TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Felis catus TNF alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA.
Amino Acid Range: Leu77-Leu233

Catalog #TNF-FM10A

Price

$375⁰⁰

Size

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Felis catus TNF alpha-Protein (active trimer) (TNF-FM10A) Summary

Product Description

Recombinant Felis catus TNF alpha-Protein (active trimer) is expressed from HEK293 with His tag at the C-terminus.It contains Leu77-Leu233.

Product Background

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Felis catus TNF alpha-Protein (active trimer) (TNF-FM10A) Specifications/Details

Protein	TNF alpha
Synonyms	APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha;TNF alpha
Accession	P19101
Species	Felis catus
Biotinylated	no
Amino Acid Range	Leu77-Leu233
Molecular Weight	The protein has a predicted MW of 18.86 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA.
	Felis catus TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Felis catus TNF alpha is greater than 95% as determined by SEC-HPLC.