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Felis catus TNF alpha-Protein (active trimer) (TNF-FM10A) Summary
Product Description |
Recombinant Felis catus TNF alpha-Protein (active trimer) is expressed from HEK293 with His tag at the C-terminus.It contains Leu77-Leu233. |
Product Background |
Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Felis catus TNF alpha-Protein (active trimer) (TNF-FM10A) Specifications/Details
Protein |
TNF alpha |
Synonyms |
APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha |
Accession |
|
Species |
Felis catus |
Biotinylated |
no |
Amino Acid Range |
Leu77-Leu233 |
Molecular Weight |
The protein has a predicted MW of 18.86 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA. |
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Felis catus TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Felis catus TNF alpha is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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