• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA.
  • Amino Acid Range: Leu77-Leu233
Catalog #TNF-FM10A
Price
Regular price $375.00
Regular price Sale price $375.00
/
Size
Avantor VWR Fisher Scientific

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Felis catus TNF alpha-Protein (active trimer) (TNF-FM10A) Summary

Product Description

 Recombinant Felis catus TNF alpha-Protein (active trimer) is expressed from HEK293 with His tag at the C-terminus.It contains Leu77-Leu233.

Product Background

 Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Felis catus TNF alpha-Protein (active trimer) (TNF-FM10A) Specifications/Details

Protein
 TNF alpha
Synonyms
 APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha
Accession
Species
 Felis catus
Biotinylated
 no
Amino Acid Range
 Leu77-Leu233
Molecular Weight
 The protein has a predicted MW of 18.86 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA. Immobilized Felis catus TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag (Cat. TNF-HM2R2) with the EC50 of 4.8ng/ml determined by ELISA.
Felis catus TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Felis catus TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Felis catus TNF alpha is greater than 95% as determined by SEC-HPLC. The purity of Felis catus TNF alpha is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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