ELISA dose-response curve showing binding of Anti-CXCR5 Antibody, hFc Tag to immobilized Human CXCR5 Nanodisc, His Tag with an EC50 of 11.7 ng/ml, measured by absorbance at 450 nm.
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Human CXCR5 Nanodisc, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Met1-Phe372
Catalog #CXR5-HM1N72
Regular price $1,444.30
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Human CXCR5 Nanodisc (CXR5-HM1N72) Summary

Product Description

Recombinant Human CXCR5 Nanodisc is expressed from HEK293 with His tag at the C-terminus (FITC-equivalent protein is fused on cytoplasmic part).It contains Met1-Phe372.

Product Background

CXCR5 is a serpentine receptor implicated in cell migration in lymphocytes and differentiation in leukocytes. It causes MAPK pathway activation and has known membrane partners for signaling. CXCR5 is also expressed in HL-60 cells, a human acute myeloid leukemia line, following treatment with all-trans retinoic acid, which induces differentiation toward a neutrophil-like state. CXCR5 is necessary for this process; differentiation was crippled in CXCR5 knockout cells and enhanced in cells ectopically expressing it.

Product Category

Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Human CXCR5 Nanodisc (CXR5-HM1N72) Specifications/Details

Protein
CXCR5
Synonyms
CXC-R5; CXCR-5; MDR-15; MDR15; CD185; BLR1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met1-Phe372
Molecular Weight
The protein has a predicted MW of 43.3 kDa.
Activity
Immobilized Human CXCR5 Nanodisc, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Expression System
HEK293
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CXCR5 Nanodisc, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test). Immobilized Human CXCR5 Nanodisc, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test).
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