FITC-Labeled Human CD5 Protein (CD5-HM101F)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized FITC-Labeled Human CD5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg25-Asn371
Catalog #CD5-HM101F
Regular price $990.00
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Product Description

Recombinant FITC-Labeled Human CD5 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Arg25-Asn371.

Product Background

CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

CD5

Synonyms

CD5 molecule; CD5; LEU1; T1; CD5 antigen

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Arg25-Asn371

Molecular Weight

The protein has a predicted MW of 39.63 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized FITC-Labeled Human CD5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Fluorophore

FITC

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized FITC-Labeled Human CD5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. Immobilized FITC-Labeled Human CD5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
FITC-Labeled Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. FITC-Labeled Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of FITC-Labeled Human CD5 is greater than 95% as determined by SEC-HPLC. The purity of FITC-Labeled Human CD5 is greater than 95% as determined by SEC-HPLC.

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