FITC-Labeled Human CD5 Protein (CD5-HM101F)

SKU: CD5-HM101F-100UG

FITC-Labeled Human CD5 Protein (CD5-HM101F)

SKU: CD5-HM101F-100UG
FITC-Labeled Human CD5 Protein (CD5-HM101F)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized FITC-Labeled Human CD5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
  • Amino Acid Range: Arg25-Asn371
Catalog #CD5-HM101F
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Size
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FITC-Labeled Human CD5 Protein (CD5-HM101F) Summary

Product Description

 Recombinant FITC-Labeled Human CD5 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Arg25-Asn371.

Product Background

 CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets

FITC-Labeled Human CD5 Protein (CD5-HM101F) Specifications/Details

Protein
 CD5
Synonyms
 CD5 molecule; CD5; LEU1; T1; CD5 antigen
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Arg25-Asn371
Molecular Weight
 The protein has a predicted MW of 39.63 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized FITC-Labeled Human CD5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Product Tag
 C-His

Fluorophore

 FITC-Labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized FITC-Labeled Human CD5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. Immobilized FITC-Labeled Human CD5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
FITC-Labeled Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. FITC-Labeled Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of FITC-Labeled Human CD5 is greater than 95% as determined by SEC-HPLC. The purity of FITC-Labeled Human CD5 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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