FITC-Labeled Human EMMPRIN/CD147 Protein (EPN-HM147F)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized FITC-Labeled Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala22-His205

Catalog: EPN-HM147F

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Product Description Recombinant FITC-Labeled Human EMMPRIN/CD147 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala22-His205.

Product Background

 CD147, also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or basigin, is expressed in a variety of cell types. It is involved in the regulation of extracellular matrix (ECM) remodeling during physiological and pathological processes including wound healing, inflammatory diseases, and cancer. CD147 is a diagnostic and therapeutic target in cancer and inflammatory diseases, either directly or indirectly, by targeting CD147 partners.

Product Category

 Recombinant Protein

Target

 EMMPRIN/CD147
Accession

Species

 Human

Amino Acid Range

 Ala22-His205
Molecular Weight The protein has a predicted MW of 21.2 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized FITC-Labeled Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Fluorophore

 FITC

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized FITC-Labeled Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
The purity of FITC-Labeled Human EMMPRIN is greater than 95% as determined by SEC-HPLC.
FITC-Labeled Human EMMPRIN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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