FITC-Labeled Human EMMPRIN/CD147 Protein (EPN-HM147F)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized FITC-Labeled Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala22-His205
Catalog #EPN-HM147F
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Product Description
 Recombinant FITC-Labeled Human EMMPRIN/CD147 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala22-His205.
Product Background
 CD147, also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or basigin, is expressed in a variety of cell types. It is involved in the regulation of extracellular matrix (ECM) remodeling during physiological and pathological processes including wound healing, inflammatory diseases, and cancer. CD147 is a diagnostic and therapeutic target in cancer and inflammatory diseases, either directly or indirectly, by targeting CD147 partners.
Product Category
 Recombinant Protein
Target
 EMMPRIN/CD147
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala22-His205
Molecular Weight
 The protein has a predicted MW of 21.2 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized FITC-Labeled Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Fluorophore
 FITC
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized FITC-Labeled Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
FITC-Labeled Human EMMPRIN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of FITC-Labeled Human EMMPRIN is greater than 95% as determined by SEC-HPLC.

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