FITC-Labeled Human Transferrin Protein (TFN-HM101F)
FITC-Labeled Human Transferrin Protein (TFN-HM101F)
FITC-Labeled Human Transferrin Protein (TFN-HM101F)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized FITC-Labeled Human Transferrin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 1.58ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Val20-Pro698
Catalog #TFN-HM101F
Regular price $990.00
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Product Description

Recombinant FITC-Labeled Human Transferrin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val20-Pro698.

Product Background

Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Transferrin

Synonyms

Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Val20-Pro698

Molecular Weight

The protein has a predicted MW of 76.3 kDa. Due to glycosylation, the protein migrates to 78-88 kDa based on Bis-Tris PAGE result.

Activity

Immobilized FITC-Labeled Human Transferrin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 1.58ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Fluorophore

FITC

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized FITC-Labeled Human Transferrin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 1.58ug/ml determined by ELISA (QC Test). Immobilized FITC-Labeled Human Transferrin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 1.58ug/ml determined by ELISA (QC Test).
FITC-Labeled Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. FITC-Labeled Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of FITC-Labeled Human Transferrin is greater than 95% as determined by SEC-HPLC. The purity of FITC-Labeled Human Transferrin is greater than 95% as determined by SEC-HPLC.

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