FITC-Labeled Human VEGF165 Protein (VEG-HM465F)
Catalog: VEG-HM465F
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| Product Description | Recombinant FITC-Labeled Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191. |
Product Background |
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
Product Category |
Recombinant Protein |
Target |
VEGF165 |
Gene/Protein ID |
P15692-4 |
Species |
Human |
Exact Sequence |
Ala27-Arg191 |
| Molecular Weight | The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-33 kDa under reduced (R) condition, 45-55 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
Activity |
Immobilized FITC-Labeled Human VEGF165, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 22.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized FITC-Labeled Human VEGF165, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 22.3ng/ml determined by ELISA. |
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The purity of FITC-Labeled Human VEGF165 is greater than 95% as determined by SEC-HPLC. |
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FITC-Labeled Human VEGF165 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. |
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