• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized FITC-Labeled Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Arg191
Catalog #VEG-HM465F
Price
Regular price $99000
Regular price Sale price $99000
/
Size
Avantor VWR Fisher Scientific

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FITC-Labeled Human VEGF165 Protein (VEG-HM465F) Summary

Product Description

 Recombinant FITC-Labeled Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.

Product Background

 Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

FITC-Labeled Human VEGF165 Protein (VEG-HM465F) Specifications/Details

Protein
 VEGF165
Synonyms
 VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165;VEGF165;Vascular endothelial growth factor-165; Vascular endothelial growth factor 165
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Arg191
Molecular Weight
 The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-33 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized FITC-Labeled Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 FITC-Labeled
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 100mM L-arginine, 8% trehalose (pH 7.4).
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized FITC-Labeled Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. Immobilized FITC-Labeled Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.
FITC-Labeled Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. FITC-Labeled Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of FITC-Labeled Human VEGF165 is greater than 90% as determined by SEC-HPLC. The purity of FITC-Labeled Human VEGF165 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...