Hamster FGF21-Protein (FGF-HM12T)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: The affinity constant of 264.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Arg29-Ser209
Catalog #FGF-HM12T
Regular price $300.00
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Product Description
Recombinant Hamster FGF21-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg29-Ser209.
Product Background
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
Product Category
Recombinant Protein
Target
FGF21
Accession
Species
Hamster
Biotinylated
no
Amino Acid Range
Arg29-Ser209
Molecular Weight
The protein has a predicted MW of 20.80 kDa. Due to glycosylation, the protein migrates to 21-30 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 264.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Hamster FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 264.50 nM as determined in SPR assay (Biacore T200).
Hamster FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Hamster FGF21 is greater than 90% as determined by SEC-HPLC.

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