Human 2B4/CD244/SLAMF4 Protein (2B4-HM101)
Human 2B4/CD244/SLAMF4 Protein (2B4-HM101)
Human 2B4/CD244/SLAMF4 Protein (2B4-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Cys22-Arg221
Catalog #2B4-HM101
Regular price $300.00
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Product Description
Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys22-Arg221.
Product Background
2B4 (CD244) is expressed by memory-phenotype CD8 T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
Product Category
Recombinant Protein
Target
2B4/CD244/SLAMF4
Synonyms
2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Cys22-Arg221
Molecular Weight
The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA.
Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human 2B4 is greater than 95% as determined by SEC-HPLC.

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