Human 2B4/CD244/SLAMF4 Protein (2B4-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Cys22-Arg221

Catalog: 2B4-HM101

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Product Description Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys22-Arg221.

Product Background

2B4 (CD244) is expressed by memory-phenotype CD8 T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.

Product Category

Recombinant Protein

Target

2B4/CD244/SLAMF4
Synonyms 2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
Accession

Species

Human

Exact Sequence

Cys22-Arg221
Molecular Weight The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA.
The purity of Human 2B4 is greater than 95% as determined by SEC-HPLC.
Human 2B4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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