- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Cys22-Arg221
Product Description |
Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys22-Arg221. |
Product Background |
2B4 (CD244) is expressed by memory-phenotype CD8 T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
2B4/CD244/SLAMF4 |
Synonyms |
2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys22-Arg221 |
Molecular Weight |
The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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