Human 4-1BB Ligand/TNFSF9 Trimer Protein (BBL-HM141)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human 4-1BB Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg71-Glu254

Catalog: BBL-HM141

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Product Description Recombinant Human 4-1BB Ligand/TNFSF9 Trimer Protein is expressed from Expi293 with His tag at the N-terminal.; It contains Arg71-Glu254.
Product Category Recombinant Protein
Target 4-1BB Ligand/TNFSF9 Trimer
Synonyms 41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Gene/Protein ID P41273
Species Human
Exact Sequence Arg71-Glu254
Molecular Weight The protein has a predicted MW of 61.8 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human 4-1BB Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human 4-1BB Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
The purity of Human 4‑1BB Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Human 4‑1BB Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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