- Express System: HEK293
- Product Tag: N-monomeric hFc
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Arg71-Glu254
Product Description |
Recombinant Human 4-1BB Ligand/TNFSF9 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Arg71-Glu254. |
Product Background |
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
4-1BB Ligand/TNFSF9 Trimer |
Synonyms |
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg71-Glu254 |
Molecular Weight |
The protein has a predicted MW of 84.3 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human 4-1BB, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-monomeric hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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