Human 4-1BB/TNFRSF9 Protein (BB4-HM541)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human 4-1BB Ligand Trimer, His Tag at 1_g/ml (100_l/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu24-Gln186

Catalog: BB4-HM541

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Product Description Recombinant Human 4-1BB/TNFRSF9 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.It contains Leu24-Gln186.
Product Category Recombinant Protein
Target 4-1BB/TNFRSF9
Synonyms CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Gene/Protein ID Q07011
Species Human
Exact Sequence Leu24-Gln186
Molecular Weight The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human 4-1BB Ligand Trimer, His Tag at 1_g/ml (100_l/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human 4-1BB Ligand Trimer, His Tag at 1_g/ml (100_l/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
Immobilized Human 4-1BB, hFc Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand, hFc Tag with the EC50 of 61ng/ml determined by ELISA.
The purity of Human 4-1BB/TNFRSF9 is greater than 95% as determined by SEC-HPLC.
Human 4-1BB/TNFRSF9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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