• Express System: HEK293
  • Product Tag: C-hFc (IgG1)-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
  • Amino Acid Range: Leu24-Gln186
Catalog #BB4-HM541
Price
Regular price $297.00
Regular price Sale price $297.00
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Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541) Summary

Product Description

 Recombinant Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu24-Gln186.

Product Background

 4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.

Non-biotinylated Human 4-1BB/TNFRSF9 Protein (BB4-HM541) Specifications/Details

Protein
 4-1BB/TNFRSF9
Synonyms
 CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu24-Gln186
Molecular Weight
 The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Non-biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.18 nM as determined in BLI assay . Loaded Non-biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.18 nM as determined in BLI assay .
The purity of Human 4-1BB is greater than 95% as determined by SEC-HPLC. The purity of Human 4-1BB is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...