Human 4-1BB/TNFRSF9 Protein (BB4-HM541)

  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human 4-1BB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu24-Gln186

Catalog: BB4-HM541

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Product Description Recombinant Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminal.It contains Leu24-Gln186.
Product Category Recombinant Protein
Target 4-1BB/TNFRSF9
Synonyms CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Gene/Protein ID Q07011
Species Human
Exact Sequence Leu24-Gln186
Molecular Weight The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human 4-1BB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human 4-1BB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA.
The purity of Human 4-1BB is greater than 95% as determined by SEC-HPLC.
Human 4-1BB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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