Bis-Tris PAGE gel image showing the molecular weight marker (lane MK) and purified Human ABO protein (lane R) under reduced conditions. The ABO protein band appears between the 70 kDa and 80 kDa markers, indicating high purity.
  • Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Ala54-Pro354
Catalog #ABO-HM201
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Size
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Human ABO Protein (ABO-HM201) Summary

Product Description

 Recombinant Human ABO Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Ala54-Pro354.

Product Background

 Histo-blood group ABH (O) antigens are major alloantigens in humans. These antigens are widely distributed in human tissues and undergo changes in expression during cellular differentiation and malignant development. The ABH antigens have been characterized as terminal disaccharide determinants which represent secondary gene products. They are synthesized in a stepwise fashion from a precursor by the action of different glycosyltransferases.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human ABO Protein (ABO-HM201) Specifications/Details

Protein
 ABO
Synonyms
 ABH
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala54-Pro354
Molecular Weight
 The protein has a predicted MW of 60.56 kDa. Due to glycosylation, the protein migrates to 62-70 kDa based on Bis-Tris PAGE result.
Product Tag
 N-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in 20mM Tris, 150mM NaCl, 500mM L- Arginine, 2mM DTT (pH 7.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human ABO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ABO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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support@kactusbio.us

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