Human ACE2/ACEH Protein (ACE-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test). The affinity constant of 11.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln18-Ser740
Catalog #ACE-HM101
Regular price $297.00
/
Size

Order now, ships by:

Product Description

Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Ser740.

Product Background

ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

ACE2/ACEH

Synonyms

ACE-2; ACEH; ACE2

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gln18-Ser740

Molecular Weight

The protein has a predicted MW of 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human ACE2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test). The affinity constant of 11.9nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200). SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200).
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test). Immobilized Human ACE2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test).
Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed