Human ACE2/ACEH Protein (ACE-HM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 11.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln18-Ser740

Catalog: ACE-HM101

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Product Description Recombinant Human ACE2/ACEH Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln18-Ser740.
Product Category Recombinant Protein
Target ACE2/ACEH
Synonyms ACE-2; ACEH; ACE2
Gene/Protein ID Q9BYF1-1
Species Human
Exact Sequence Gln18-Ser740
Molecular Weight The protein has a predicted MW of 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 11.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200).
The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC
Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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