Human ACE2/ACEH Protein (ACE-HM401)
Human ACE2/ACEH Protein (ACE-HM401)
Human ACE2/ACEH Protein (ACE-HM401)
Human ACE2/ACEH Protein (ACE-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln18-Ser740
Catalog #ACE-HM401
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Product Description

 Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.

Product Background

 ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.

Product Category

 Recombinant Protein

Protein

 ACE2/ACEH

Synonyms

 ACE-2; ACEH; ACE2

Accession

Species

 Human

Biotinylated

 no

Amino Acid Range

 Gln18-Ser740

Molecular Weight

 The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.

Activity

 Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His-Avi

Expression System

 HEK293

Purity

 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).
Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC

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