Human ACE2/ACEH Protein (ACE-HM401)
Human ACE2/ACEH Protein (ACE-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln18-Ser740
Catalog #ACE-HM401
Regular price $270.00
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Product Description
Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.
Product Background
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Product Category
Recombinant Protein
Target
ACE2/ACEH
Synonyms
ACE-2; ACEH; ACE2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln18-Ser740
Molecular Weight
The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).
Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC

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