Human ACE2/ACEH Protein (ACE-HM401)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln18-Ser740

Catalog: ACE-HM401

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Product Description	Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.
Product Background	ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Product Category	Recombinant Protein

Target	ACE2/ACEH
Synonyms	ACE-2; ACEH; ACE2
Gene/Protein ID	Q9BYF1-1
Species	Human
Exact Sequence	Gln18-Ser740
Molecular Weight	The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).
	Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
	The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC
	Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.