Human ADAM8 Protein (ADM-HM108)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ADAM8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ile17-Pro655

Catalog: ADM-HM108

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human ADAM8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile17-Pro655.
Product Background	A Disintegrin And Metalloproteinase (ADAM) proteases constitute a family of multifunctional, membrane-bound proteins with traditional sheddase functions. Their protumorigenic potential has been attributed to both, essential (ADAM10 and ADAM17) and 'dispensable' ADAM proteases (ADAM8, 9, 12, 15, and 19). Of specific interest in this review is the ADAM proteinase ADAM8 that has been identified as a significant player in aggressive malignancies including breast, pancreatic, and brain cancer.
Product Category	Recombinant Protein

Target	ADAM8
Synonyms	ADAM 8; Ms2; Adam8; CD156a; MGC134985
Gene/Protein ID	P78325
Species	Human
Exact Sequence	Ile17-Pro655
Molecular Weight	The protein has a predicted MW of 70.9 kDa. The protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human ADAM8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human ADAM8, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA.
	Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >1 pmol/min/µg
	The purity of Human ADAM8 is greater than 95% as determined by SEC-HPLC.
	Human ADAM8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.