Name: Human ADAM8 Protein (ADM-HM108)
Brand: KactusBio
SKU: ADM-HM108-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ADAM8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >1 pmol/min/µg.
Amino Acid Range: Ile17-Pro655

Catalog #ADM-HM108

Size

100UG

500UG

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Human ADAM8 Protein (ADM-HM108) Summary

Product Description	Recombinant Human ADAM8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile17-Pro655.
Product Background	A Disintegrin And Metalloproteinase (ADAM) proteases constitute a family of multifunctional, membrane-bound proteins with traditional sheddase functions. Their protumorigenic potential has been attributed to both, essential (ADAM10 and ADAM17) and 'dispensable' ADAM proteases (ADAM8, 9, 12, 15, and 19). Of specific interest in this review is the ADAM proteinase ADAM8 that has been identified as a significant player in aggressive malignancies including breast, pancreatic, and brain cancer.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human ADAM8 Protein (ADM-HM108) Specifications/Details

Protein	ADAM8
Synonyms	ADAM 8; Ms2; Adam8; CD156a; MGC134985
Accession	P78325
Species	Human
Biotinylated	no
Amino Acid Range	Ile17-Pro655
Molecular Weight	The protein has a predicted MW of 70.9 kDa. The protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ADAM8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >1 pmol/min/µg.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human ADAM8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
	Human ADAM8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-ADAM8 Ab., hFc Tag on ProA-Biosensor can bind Human ADAM8, His Tag with an affinity constant of 0.87 nM as determined in BLI assay .
	The purity of Human ADAM8 is greater than 95% as determined by SEC-HPLC.

Other Available Proteins:

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