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Human ADAM8 Protein, Ultra Low Endotoxin (ADM-HM108-UL) Summary
Product Description |
Recombinant Human ADAM8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile17-Pro655. |
Product Background |
A Disintegrin And Metalloproteinase (ADAM) proteases constitute a family of multifunctional, membrane-bound proteins with traditional sheddase functions. Their protumorigenic potential has been attributed to both, essential (ADAM10 and ADAM17) and 'dispensable' ADAM proteases (ADAM8, 9, 12, 15, and 19). Of specific interest in this review is the ADAM proteinase ADAM8 that has been identified as a significant player in aggressive malignancies including breast, pancreatic, and brain cancer. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human ADAM8 Protein, Ultra Low Endotoxin (ADM-HM108-UL) Specifications/Details
Protein |
ADAM8 |
Synonyms |
ADAM 8; Ms2; Adam8; CD156a; MGC134985 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile17-Pro655 |
Molecular Weight |
The protein has a predicted MW of 70.9 kDa. The protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ADAM8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >1 pmol/min/µg. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human ADAM8, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. |
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Human ADAM8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-ADAM8 Ab., hFc Tag on ProA-Biosensor can bind Human ADAM8, His Tag with an affinity constant of 0.87 nM as determined in BLI assay . |
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The purity of Human ADAM8 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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