Product Description |
Recombinant Human Aggrecan Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala17-Leu721. |
Product Background |
Aggrecan is a large proteoglycan that forms giant hydrated aggregates with hyaluronan in the extracellular matrix (ECM). The extraordinary resistance of these aggregates to compression explains their abundance in articular cartilage of joints where they ensure adequate load-bearing. In the brain, they provide mechanical buffering and contribute to formation of perineuronal nets, which regulate synaptic plasticity. Aggrecan is also present in cardiac jelly, developing heart valves, and blood vessels during cardiovascular development. Whereas aggrecan is essential for skeletal development, its function in the developing cardiovascular system remains to be fully elucidated. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Aggrecan |
Synonyms |
ACAN; AGC1; CSPG1; MSK16 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala17-Leu721 |
Molecular Weight |
The protein has a predicted MW of 78.86 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human Aggrecan on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Aggrecan is greater than 95% as determined by SEC-HPLC. |
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