Name: Human ALK-1/ACVRL1 Protein (ALK-HM101)
Brand: KactusBio
SKU: ALK-HM101-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Asp22-Gln118

Catalog #ALK-HM101

Size

100UG

500UG

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Product Description	Recombinant Human ALK-1/ACVRL1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp22-Gln118.
Product Background	Activin receptor-like kinase 1 (ALK1)-mediated endothelial cell signalling in response to bone morphogenetic protein 9 (BMP9) and BMP10 is of significant importance in cardiovascular disease and cancer. Structural analyses reveal a tripartite recognition mechanism that defines BMP9 and BMP10 specificity for ALK1, and predict that crossveinless 2 is not an inhibitor of BMP9, which is confirmed by experimental evidence.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	ALK-1/ACVRL1
Synonyms	ACVRLK1; ORW2; ALK1; ALK-1; EC 2.7.11; HHT; HHT2; SKR3; TSR-I; ACVRL1; Activin RLI;Activin RL1
Accession	P37023
Species	Human
Biotinylated	no
Amino Acid Range	Asp22-Gln118
Molecular Weight	The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test).
	Human ALK-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is <50 ng/mL in the presence of 2 ng/mL of recombinant human BMP9 (Cat.GDF-HM002).
	The purity of Human ALK-1 is greater than 95% as determined by SEC-HPLC.