Human ALK-1/ACVRL1 Protein (ALK-HM101)
Human ALK-1/ACVRL1 Protein (ALK-HM101)
Human ALK-1/ACVRL1 Protein (ALK-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Asp22-Gln118
Catalog #ALK-HM101
Regular price $264.00
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Product Description

Recombinant Human ALK-1/ACVRL1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp22-Gln118.

Product Background

Activin receptor-like kinase 1 (ALK1)-mediated endothelial cell signalling in response to bone morphogenetic protein 9 (BMP9) and BMP10 is of significant importance in cardiovascular disease and cancer. Structural analyses reveal a tripartite recognition mechanism that defines BMP9 and BMP10 specificity for ALK1, and predict that crossveinless 2 is not an inhibitor of BMP9, which is confirmed by experimental evidence.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

ALK-1/ACVRL1

Synonyms

ACVRLK1; ORW2; ALK1; ALK-1; EC 2.7.11; HHT; HHT2; SKR3; TSR-I; ACVRL1; Activin RLI;Activin RL1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Asp22-Gln118

Molecular Weight

The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test).
Human ALK-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ALK-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is <50 ng/mL in the presence of 2 ng/mL of recombinant human BMP9 (Cat.GDF-HM002). Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is <50 ng/mL in the presence of 2 ng/mL of recombinant human BMP9 (Cat.GDF-HM002).
The purity of Human ALK-1 is greater than 95% as determined by SEC-HPLC. The purity of Human ALK-1 is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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