Human ALK-1/Activin RL1 Protein (ALK-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp22-Gln118

Catalog: ALK-HM101

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Product Description	Recombinant Human ALK-1/Activin RL1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp22-Gln118.
Product Background	Activin receptor-like kinase 1 (ALK1)-mediated endothelial cell signalling in response to bone morphogenetic protein 9 (BMP9) and BMP10 is of significant importance in cardiovascular disease and cancer. Structural analyses reveal a tripartite recognition mechanism that defines BMP9 and BMP10 specificity for ALK1, and predict that crossveinless 2 is not an inhibitor of BMP9, which is confirmed by experimental evidence.
Product Category	Recombinant Protein

Target	ALK-1/Activin RL1
Synonyms	ACVRLK1; ORW2; ALK1; ALK-1; EC 2.7.11; HHT; HHT2; SKR3; TSR-I; ACVRL1
Accession	P37023
Species	Human
Amino Acid Range	Asp22-Gln118
Molecular Weight	The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 9.6ng/ml determined by ELISA.
	The purity of Human ALK-1 is greater than 95% as determined by SEC-HPLC.
	Human ALK-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.