Human ALK-1/Activin RL1 Protein (ALK-HM101)
Human ALK-1/Activin RL1 Protein (ALK-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp22-Gln118
Catalog #ALK-HM101
Regular price $240.00
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Product Description
Recombinant Human ALK-1/Activin RL1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp22-Gln118.
Product Background
Activin receptor-like kinase 1 (ALK1)-mediated endothelial cell signalling in response to bone morphogenetic protein 9 (BMP9) and BMP10 is of significant importance in cardiovascular disease and cancer. Structural analyses reveal a tripartite recognition mechanism that defines BMP9 and BMP10 specificity for ALK1, and predict that crossveinless 2 is not an inhibitor of BMP9, which is confirmed by experimental evidence.
Product Category
Recombinant Protein
Target
ALK-1/Activin RL1
Synonyms
ACVRLK1; ORW2; ALK1; ALK-1; EC 2.7.11; HHT; HHT2; SKR3; TSR-I; ACVRL1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asp22-Gln118
Molecular Weight
The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 9.6ng/ml determined by ELISA.
Human ALK-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ALK-1 is greater than 95% as determined by SEC-HPLC.

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