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Human ALK-1/ACVRL1 Protein, Ultra Low Endotoxin (ALK-HM101-UL) Summary
Product Description |
Recombinant Human ALK-1/ACVRL1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp22-Gln118. |
Product Background |
Activin receptor-like kinase 1 (ALK1)-mediated endothelial cell signalling in response to bone morphogenetic protein 9 (BMP9) and BMP10 is of significant importance in cardiovascular disease and cancer. Structural analyses reveal a tripartite recognition mechanism that defines BMP9 and BMP10 specificity for ALK1, and predict that crossveinless 2 is not an inhibitor of BMP9, which is confirmed by experimental evidence. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human ALK-1/ACVRL1 Protein, Ultra Low Endotoxin (ALK-HM101-UL) Specifications/Details
Protein |
ALK-1/ACVRL1 |
Synonyms |
ACVRLK1; ORW2; ALK1; ALK-1; EC 2.7.11; HHT; HHT2; SKR3; TSR-I; ACVRL1; Activin RLI;Activin RL1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp22-Gln118 |
Molecular Weight |
The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). |
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Human ALK-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is <50 ng/mL in the presence of 2 ng/mL of recombinant human BMP9 (Cat.GDF-HM002). |
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The purity of Human ALK-1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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