Product Description |
Recombinant Human Alkaline Phosphatase (Germ type)/ALPG Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Ile20-Asp503. |
Product Background |
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
Product Category |
Recombinant Protein |
Target |
Alkaline Phosphatase (Germ type)/ALPG |
Synonyms |
Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile20-Asp503 |
Molecular Weight |
The protein has a predicted MW of 53.67 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Alkaline Phosphatase (Germ type), His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human Alkaline Phosphatase (Germ type), His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA. |
|
|
Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Alkaline Phosphatase (Germ type) is greater than 95% as determined by SEC-HPLC. |
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