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Human Alkaline Phosphatase (Placental type) /ALPP Protein, Ultra Low Endotoxin (APE-HM102-UL) Summary
Product Description |
Recombinant Human Alkaline Phosphatase (Placental type) /ALPP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asp506. |
Product Background |
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Alkaline Phosphatase (Placental type) /ALPP Protein, Ultra Low Endotoxin (APE-HM102-UL) Specifications/Details
Protein |
Alkaline Phosphatase (Placental type)/ALPP |
Synonyms |
Alkaline phosphatase; PLAP-1; ALPP; PLAP |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile23-Asp506 |
Molecular Weight |
The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 63-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Alkaline Phosphatase (Placental type), His Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 12000 pmol/min/µg. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Alkaline Phosphatase (Placental type), His Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. |
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Human Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Human Alkaline Phosphatase (Placental type), His Tag with an affinity constant of 0.56 nM as determined in BLI assay. |
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The purity of Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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