Human Alkaline Phosphatase Protein (APE-HM102)
Catalog: APE-HM102
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| Product Description | Recombinant Human Alkaline Phosphatase Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Ile23-Asp506. |
| Product Category | Recombinant Protein |
| Target | Alkaline phosphatase |
| Synonyms | IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP |
| Gene/Protein ID | P05187 |
| Species | Human |
| Exact Sequence | Ile23-Asp506 |
| Molecular Weight | The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Alkaline Phosphatase, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 17.1ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with blue ice or dry ice. |
| Formulation | Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Alkaline Phosphatase, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 17.1ng/ml determined by ELISA. |
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The purity of Human Alkaline Phosphatase is greater than 95% as determined by SEC-HPLC. |
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Human Alkaline Phosphatase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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