Human Alkaline Phosphatase (Placental type)/ALPP Protein (APE-HM102)
Human Alkaline Phosphatase (Placental type)/ALPP Protein (APE-HM102)
Human Alkaline Phosphatase (Placental type)/ALPP Protein (APE-HM102)
Human Alkaline Phosphatase (Placental type)/ALPP Protein (APE-HM102)
Human Alkaline Phosphatase (Placental type)/ALPP Protein (APE-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human Alkaline Phosphatase (Placental type), His Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ile23-Asp506
Catalog #APE-HM102
Regular price $300.00
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Product Description
Recombinant Human Alkaline Phosphatase (Placental type)/ALPP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asp506.
Product Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category
Recombinant Protein
Target
Alkaline Phosphatase (Placental type)/ALPP
Synonyms
IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ile23-Asp506
Molecular Weight
The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 63-68 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Alkaline Phosphatase (Placental type), His Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Alkaline Phosphatase (Placental type), His Tag at 1ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA (QC Test).
Human Alkaline Phosphatase (Placental type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.
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