Human Alkaline Phosphatase Protein (APE-HM102)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human Alkaline Phosphatase, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 17.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile23-Asp506

Catalog: APE-HM102

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Product Description Recombinant Human Alkaline Phosphatase Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Ile23-Asp506.
Product Category Recombinant Protein
Target Alkaline phosphatase
Synonyms IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP
Gene/Protein ID P05187
Species Human
Exact Sequence Ile23-Asp506
Molecular Weight The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Alkaline Phosphatase, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 17.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with blue ice or dry ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Alkaline Phosphatase, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 17.1ng/ml determined by ELISA.
The purity of Human Alkaline Phosphatase is greater than 95% as determined by SEC-HPLC.
Human Alkaline Phosphatase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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