Human Alkaline phosphatase Protein (Intestinal type) (APE-HM101)

  • Express System: Expi293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.96 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val20-Asp503

Catalog: APE-HM101

$469.00
Size:

Order now get it on

Product Description Recombinant Human Alkaline phosphatase Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.It contains Val20-Asp503.
Product Category Recombinant Protein
Target Alkaline phosphatase
Synonyms IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP
Gene/Protein ID P09923
Species Human
Exact Sequence Val20-Asp503
Molecular Weight The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.96 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline Phosphatase, His Tag with an affinity constant of 0.96 μM as determined in SPR assay (Biacore T200).
The purity of Human Alkaline phosphatase is greater than 95% as determined by SEC-HPLC.
Human Alkaline phosphatase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in