Human Alkaline phosphatase Protein (Intestinal type) (APE-HM101)

  • Express System: Expi293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.96 _M as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val20-Asp503

Catalog: APE-HM101

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Product Description Recombinant Human Alkaline phosphatase Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.It contains Val20-Asp503.
Product Category Recombinant Protein
Target Alkaline phosphatase
Synonyms IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP
Gene/Protein ID P09923
Species Human
Exact Sequence Val20-Asp503
Molecular Weight The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.96 _M as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline Phosphatase, His Tag with an affinity constant of 0.96 _M as determined in SPR assay (Biacore T200).
The purity of Human Alkaline phosphatase is greater than 95% as determined by SEC-HPLC.
Human Alkaline phosphatase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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