Product Description |
Recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val20-Asp503. |
Product Background |
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
Product Category |
Recombinant Protein |
Target |
Alkaline phosphatase (Intestinal type) /ALPI |
Synonyms |
IAP; Akp2; ALPI; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val20-Asp503 |
Molecular Weight |
The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.58 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 uM as determined in SPR assay (Biacore T200). |
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Human Alkaline phosphatase (Intestinal type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Alkaline phosphatase (Intestinal type) is greater than 95% as determined by SEC-HPLC. |
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