Name: Human Alkaline phosphatase (Intestinal type) /ALPI Protein (APE-HM101)
Brand: KactusBio
SKU: APE-HM101-100UG
Price: 375.0 USD
Availability: InStock

Human Alkaline phosphatase (Intestinal type) /ALPI Protein (APE-HM101)

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 uM as determined in SPR assay (Biacore T200).

Human Alkaline phosphatase (Intestinal type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Alkaline phosphatase (Intestinal type) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 1.58 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Val20-Asp503

Catalog #APE-HM101

Size

100UG

500UG

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Product Description	Recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val20-Asp503.
Product Background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category	Recombinant Protein / Antibody discovery

Protein	Alkaline phosphatase (Intestinal type) /ALPI
Synonyms	IAP; Akp2; ALPI; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP
Accession	P09923
Species	Human
Biotinylated	no
Amino Acid Range	Val20-Asp503
Molecular Weight	The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 1.58 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 uM as determined in SPR assay (Biacore T200).
	Human Alkaline phosphatase (Intestinal type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Alkaline phosphatase (Intestinal type) is greater than 95% as determined by SEC-HPLC.