Human Alkaline phosphatase (Intestinal type) /ALPI Protein (APE-HM101)
Catalog: APE-HM101
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| Product Description | Recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val20-Asp503. |
| Product Category | Recombinant Protein |
| Target | Alkaline phosphatase (Intestinal type) /ALPI |
| Synonyms | IAP; Akp2; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; HOPS; MGC161443; TNAP; TNSALP |
| Gene/Protein ID | P09923 |
| Species | Human |
| Exact Sequence | Val20-Asp503 |
| Molecular Weight | The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 1.58 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His-Flag |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 uM as determined in SPR assay (Biacore T200). |
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The purity of Human Alkaline phosphatase (Intestinal type) is greater than 95% as determined by SEC-HPLC. |
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Human Alkaline phosphatase (Intestinal type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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