• Express System: E.coli
  • Product Tag: No Tag
  • Activity: Immobilized Human Alpha Synuclein Pre-formed Fibrils, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Prasinezumab, hFc Tag with the EC50 of 0.5ng/ml determined by ELISA.
  • Amino Acid Range: Met1-Ala140
Catalog #ALS-HE00F
Price
Regular price $30000
Regular price Sale price $30000
/
Size
Avantor VWR Fisher Scientific

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Human Alpha Synuclein Pre-formed Fibrils Protein (ALS-HE00F) Summary

Product Description

 Recombinant Human Alpha Synuclein Pre-formed Fibrils Protein is expressed from E.coli without tag.It contains Met1-Ala140.

Product Background

 Abnormal forms of α-syn trigger selective and progressive neuronal death through mitochondrial impairment, lysosomal dysfunction, and alteration of calcium homeostasis not only in PD but also in other α-syn-related neurodegenerative disorders such as dementia with Lewy bodies, multiple system atrophy, pure autonomic failure, and REM sleep behavior disorder.

Human Alpha Synuclein Pre-formed Fibrils Protein (ALS-HE00F) Specifications/Details

Protein
 Alpha Synuclein
Synonyms
 Alpha-synuclein; NACP; SNCA; PARK1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Ala140
Molecular Weight
 The Alpha Synuclein monomer protein has a predicted MW of 14.5 kDa.
Activity
 Immobilized Human Alpha Synuclein Pre-formed Fibrils, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Prasinezumab, hFc Tag with the EC50 of 0.5ng/ml determined by ELISA.
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 E.coli
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C. Do not store Pre-formed Fibrils at 4°C.； Recommend to aliquot the Pre-formed Fibrils protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Alpha Synuclein Pre-formed Fibrils, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Prasinezumab, hFc Tag with the EC50 of 0.5ng/ml determined by ELISA. Immobilized Human Alpha Synuclein Pre-formed Fibrils, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Prasinezumab, hFc Tag with the EC50 of 0.5ng/ml determined by ELISA.
Thioflavin T emission intensity increases progressively over time upon incubation of Alpha Synuclein Monomer with Alpha Synuclein Pre-formed Fibrils. Fluorescence signals were detected with a microplate reader at an excitation wavelength of 450nm and an emission wavelength of 485nm. Thioflavin T emission intensity increases progressively over time upon incubation of Alpha Synuclein Monomer with Alpha Synuclein Pre-formed Fibrils. Fluorescence signals were detected with a microplate reader at an excitation wavelength of 450nm and an emission wavelength of 485nm.
Negative-stain transmission electron microscopy (TEM) images of Human Alpha Synuclein Pre-formed Fibrils, No Tag. Distinct fibrillar structures are visible in TEM micrographs of this product. Negative-stain transmission electron microscopy (TEM) images of Human Alpha Synuclein Pre-formed Fibrils, No Tag. Distinct fibrillar structures are visible in TEM micrographs of this product.
Other Available Proteins:
Finding available proteins...