• Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Alpha Synuclein, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Prasinezumab, hFc Tag with the EC50 of 0.4ng/ml determined by ELISA.
  • Amino Acid Range: Met1-Ala140
Catalog #ALS-HE001
Price
Regular price $8250
Regular price Sale price $8250
/
Size
Avantor VWR Fisher Scientific

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Human Alpha Synuclein Protein (ALS-HE001) Summary

Product Description

 Recombinant Human Alpha Synuclein Protein is expressed from E.coli without tag.It contains Met1-Ala140.

Product Background

 Alpha-synuclein (α-synuclein) is a small, acidic protein containing 140 amino acids, highly expressed in the brain and primarily localized in the presynaptic terminals. It is found in high concentrations in Lewy Bodies, proteinaceous aggregates that constitute a typical histopathologic hallmark of Parkinson's disease.

Human Alpha Synuclein Protein (ALS-HE001) Specifications/Details

Protein
 Alpha Synuclein
Synonyms
 Alpha-synuclein; NACP; SNCA; PARK1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Ala140
Molecular Weight
 The protein has a predicted MW of 14.5 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human Alpha Synuclein, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Prasinezumab, hFc Tag with the EC50 of 0.4ng/ml determined by ELISA.
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Alpha Synuclein, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Prasinezumab, hFc Tag with the EC50 of 0.4ng/ml determined by ELISA. Immobilized Human Alpha Synuclein, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Prasinezumab, hFc Tag with the EC50 of 0.4ng/ml determined by ELISA.
Human Alpha Synuclein on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Alpha Synuclein on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Alpha Synuclein is greater than 95% as determined by SEC-HPLC. The purity of Human Alpha Synuclein is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...