Product Description |
Recombinant Human AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Ala476. |
Product Background |
Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. Despite that AMCase expression in the inflammation is under investigation, little is known regarding its regulation during macrophages' full maturation and polarization. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
AMCase/CHIA |
Synonyms |
Acidic mammalian chitinase; AMCase; CHIA; CHIT2; EC 3.2.1.14; Chia1; YNL |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr22-Ala476 |
Molecular Weight |
The protein has a predicted MW of 51.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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