Human AMCase/CHIA Protein (AMC-HM101)
Human AMCase/CHIA Protein (AMC-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Tyr22-Ala476
Catalog #AMC-HM101
Regular price $270.00
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Product Description

Recombinant Human AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Ala476.

Product Background

Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. Despite that AMCase expression in the inflammation is under investigation, little is known regarding its regulation during macrophages' full maturation and polarization.

Product Category

Recombinant Protein / Antibody discovery

Protein

AMCase/CHIA

Synonyms

Acidic mammalian chitinase; AMCase; CHIA; CHIT2; EC 3.2.1.14; Chia1; YNL

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Tyr22-Ala476

Molecular Weight

The protein has a predicted MW of 51.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human AMCase/CHIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human AMCase/CHIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human AMCase/CHIA is greater than 95% as determined by SEC-HPLC. The purity of Human AMCase/CHIA is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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