Product Description |
Recombinant Human AMIGO2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val40-Thr398. |
Product Background |
Bromodomain and extraterminal domain inhibitors (BETi) represent promising therapeutic agents for metastatic melanoma, yet their mechanism of action remains unclear. Here we interrogated the transcriptional effects of BETi and identified AMIGO2, a transmembrane molecule, as a BET target gene essential for melanoma cell survival. AMIGO2 is upregulated in melanoma cells and tissues compared to human melanocytes and nevi, and AMIGO2 silencing in melanoma cells induces G1/S arrest followed by apoptosis. |
Product Category |
Recombinant Protein |
Target |
AMIGO2 |
Synonyms |
Amphotein-induced protein 2; AMIGO-2; Alivin-1; DEGA; AMIGO2; ALI1; alivin 1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val40-Thr398 |
Molecular Weight |
The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human AMIGO2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human AMIGO2 is greater than 95% as determined by SEC-HPLC. |
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