Human ANGPT2/Angiopoietin-2 Protein (APN-HM401)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human ANGPT2, His Tag at 2μg/ml (100μl/well). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Lys275-Phe496

Catalog: APN-HM401

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Product Description Recombinant Human ANGPT2/Angiopoietin-2 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Lys275-Phe496.
Product Category Recombinant Protein
Target ANGPT2/Angiopoietin-2
Synonyms AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
Gene/Protein ID O15123-1
Species Human
Exact Sequence Lys275-Phe496
Molecular Weight The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ANGPT2, His Tag at 2μg/ml (100μl/well). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ANGPT2, His Tag at 2μg/ml (100μl/well). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
Immobilized Human ANGPT2, His Tag at 2μg/ml (100μl/well). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 12.5ng/ml / 9.3ng/ml / 9.3ng/ml determined by ELISA.
Human ANGPT2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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