Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM103)
Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser17-Glu460
Catalog #ANG-HM103
Regular price $203.00
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Product Description
Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser17-Glu460.
Product Background
ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.
Product Category
Recombinant Protein
Target
ANGPTL3/Angiopoietin-like 3
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser17-Glu460
Molecular Weight
The protein has a predicted MW of 52.90 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human ANGPTL3, His Tag immobilized on CM5 Chip can bind Anti-ANGPTL3 Antibody, hFc Tag with an affinity constant of 0.56 nM as determined in SPR assay (Biacore T200).
Immobilized Human ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA (QC Test).
Human ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ANGPTL3 is greater than 90% as determined by SEC-HPLC.

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