Human ANGPTL4/Angiopoietin-like 4 Protein (ANG-HM1L4)
Human ANGPTL4/Angiopoietin-like 4 Protein (ANG-HM1L4)
Human ANGPTL4/Angiopoietin-like 4 Protein (ANG-HM1L4)
Human ANGPTL4/Angiopoietin-like 4 Protein (ANG-HM1L4)
Human ANGPTL4/Angiopoietin-like 4 Protein (ANG-HM1L4)
Human ANGPTL4/Angiopoietin-like 4 Protein (ANG-HM1L4)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 51.66 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Pro166-Ser406
Catalog #ANG-HM1L4
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Product Description
 Recombinant Human ANGPTL4/Angiopoietin-like 4 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro166-Ser406.
Product Background
 Candidates for this common regulatory system include signals mediated by peroxisome proliferator-activated regulator and its response factor, angiopoietin-like 4. The expression and bioactivity of angiopoietin-like 4, an adipocytokine that was originally reported to have an angiogenic function, have been detected not only in the vascular system and adipose tissue but also in rheumatoid joints.
Product Category
 Recombinant Protein
Target
 ANGPTL4/Angiopoietin-like 4
Synonyms
 ANGPTL4; ARP4; FIAF; HFARP; Angiopoietin like 4; NGPTL2; NL2; PGAR; pp1158; ANG-3; ANG4; AGP4; ANG3; ANG-3; ANG-4;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro166-Ser406
Molecular Weight
 The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 51.66 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human ANGPTL4, His Tag with an affinity constant of 51.66 nM as determined in SPR assay (Biacore T200) (QC Test).
Human Angiopoietin-like 4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Angiopoietin-like 4 is greater than 95% as determined by SEC-HPLC.

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