Name: Human APCDD1 Protein (APD-HM101)
Brand: KactusBio
SKU: APD-HM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human APCDD1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu27-His492

Catalog #APD-HM101

Size

100UG

500UG

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Product Description	Recombinant Human APCDD1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu27-His492.
Product Background	Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	APCDD1
Synonyms	Apcdd1; Drapc1; B7323; FP7019; HHS; HTS; HYPT1
Accession	Q8J025
Species	Human
Biotinylated	no
Amino Acid Range	Leu27-His492
Molecular Weight	The protein has a predicted MW of 54.4 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human APCDD1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human APCDD1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
	Human APCDD1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.