Product Description |
Recombinant Human APCDD1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu27-His492. |
Product Background |
Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE. |
Product Category |
Recombinant Protein |
Protein |
APCDD1 |
Synonyms |
Apcdd1; Drapc1; B7323; FP7019; HHS; HTS; HYPT1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu27-His492 |
Molecular Weight |
The protein has a predicted MW of 54.4 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human APCDD1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human APCDD1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APCDD1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). |
|
|
Human APCDD1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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