Human APCDD1 Protein (APD-HM201)
Human APCDD1 Protein (APD-HM201)
Human APCDD1 Protein (APD-HM201)
Human APCDD1 Protein (APD-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human APCDD1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-APCDD1 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu27-His492
Catalog #APD-HM201
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Product Description
 Recombinant Human APCDD1 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Leu27-His492.
Product Background
 Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE.
Product Category
 Recombinant Protein
Target
 APCDD1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu27-His492
Molecular Weight
 The protein has a predicted MW of 80.06 kDa. Due to glycosylation, the protein migrates to 82-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human APCDD1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-APCDD1 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human APCDD1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-APCDD1 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA.
Human APCDD1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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