Human APLN Protein (APN-HM201)
Catalog: APN-HM201
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| Product Description | Recombinant Human APLN Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gly23-Phe77. |
| Product Category | Recombinant Protein |
| Target | APLN |
| Synonyms | Apelin；APJ endogenous ligand；APEL |
| Gene/Protein ID | Q9ULZ1 |
| Species | Human |
| Exact Sequence | Gly23-Phe77 |
| Molecular Weight | The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human APLN, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human APLN, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA. |
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Human APLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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