Human APLN Protein (APN-HM201)
Human APLN Protein (APN-HM201)
Human APLN Protein (APN-HM201)
Human APLN Protein (APN-HM201)
Human APLN Protein (APN-HM201)
Human APLN Protein (APN-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human APLN, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly23-Phe77
Catalog #APN-HM201
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Product Description
 Recombinant Human APLN Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly23-Phe77.
Product Background
 Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages. Additionally, due to the widespread distribution of the CVD, more effective therapeutic strategies need to be developed to overcome the related complications.
Product Category
 Recombinant Protein
Target
 APLN
Synonyms
 Apelin；APJ endogenous ligand；APEL
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly23-Phe77
Molecular Weight
 The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human APLN, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human APLN, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA.
Human APLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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