|Recombinant Human APLN Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly23-Phe77.
|Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages. Additionally, due to the widespread distribution of the CVD, more effective therapeutic strategies need to be developed to overcome the related complications.
|Apelin；APJ endogenous ligand；APEL
|The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
|Immobilized Human APLN, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 22.2ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.