Name: Human APLN Protein (APN-HM201)
Brand: KactusBio
SKU: APN-HM201-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human APLN, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly23-Phe77

Catalog #APN-HM201

Size

100UG

500UG

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Product Description	Recombinant Human APLN Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly23-Phe77.
Product Background	Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages. Additionally, due to the widespread distribution of the CVD, more effective therapeutic strategies need to be developed to overcome the related complications.
Product Category	Recombinant Protein / Antibody discovery

Protein	APLN
Synonyms	Apelin;APJ endogenous ligand;APEL
Accession	Q9ULZ1
Species	Human
Biotinylated	no
Amino Acid Range	Gly23-Phe77
Molecular Weight	The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human APLN, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human APLN, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA.
	Human APLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.