Human APLN Protein (APN-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human APLN, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly23-Phe77

Catalog: APN-HM201

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Product Description Recombinant Human APLN Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gly23-Phe77.
Product Category Recombinant Protein
Target APLN
Synonyms Apelin;APJ endogenous ligand;APEL
Gene/Protein ID Q9ULZ1
Species Human
Exact Sequence Gly23-Phe77
Molecular Weight The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human APLN, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human APLN, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA.
Human APLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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