Human APOA1 Protein (APO-HM201) Summary
Product Description |
Recombinant Human APOA1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Asp25-Gln267. |
Product Background |
Apolipoprotein A1 (ApoA1) is a main protein moiety in high-density lipoprotein (HDL) particles. Generally, ApoA1 and HDL are considered as atheroprotective. In prooxidant and inflammatory microenvironment in the vicinity to the atherosclerotic lesion, ApoA1/HDL are subjected to modification. The chemical modifications such as oxidation, nitration, etc result in altering native architecture of ApoA1 toward dysfunctionality and abnormality. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human APOA1 Protein (APO-HM201) Specifications/Details
Protein |
APOA1 |
Synonyms |
Apo-AI; ProapoA-I; Apoa1; Apolipoprotein A-I; APOA1; MGC117399; Alp-1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp25-Gln267 |
Molecular Weight |
The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human APOA1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human APOA1 is greater than 95% as determined by SEC-HPLC. |
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